Understanding BET and Limulus Amebocyte Lysate (LAL) Testing
The BET test, also know as the Limulus Amebocyte Lysate (LAL) test, is an in vitro assay used for detection and quantitation of bacterial endotoxin. LAL is an aqueous extract of the blood cells of horseshoe crabs which forms a clot or change in color, depending on the technique, in the presence of bacterial endotoxin. Validated test methods include both the gel clot and chromogenic methods. Inhibition / enhancement testing is performed prior to routine LAL testing to demonstrate validity of the LAL test.
Testing Capabilities and Specifications
| Test Name | Sample Needed | Turn-Around Time |
|---|---|---|
| LAL Gel Clot: Routine | Lot size dependent; 1-10 units | 1 week |
| LAL Gel Clot: Inhibition and Enhancement (I&E) | Three (3) lots of product or product family | 1 week |
| Chromogenic Assay: Routine | Lot size dependent; 1-10 units | 1 week |
| Chromogenic Assay: Inhibition and Enhancement (I&E) | Three (3) lots of product or product family | 1 week |