Bacterial Endotoxin (LAL) Testing

In vitro assays to detect and quantify bacterial endotoxins

Limulus Amebocyte Lysate (LAL) Testing

The Limulus Amebocyte Lysate (LAL) test is an in vitro assay used for detection and quantitation of bacterial endotoxin. LAL is an aqueous extract of the blood cells of horseshoe crabs which forms a clot or change in color, depending on the technique, in the presence of bacterial endotoxin. Validated test methods include both the gel clot and chromogenic methods. Inhibition / enhancement testing is performed prior to routine LAL testing to demonstrate validity of the LAL test.

Testing Capabilities and Specifications

Test NameSample NeededTurn-Around Time
LAL Gel Clot: RoutineLot size dependent; 1-10 units1 week
LAL Gel Clot: Inhibition and Enhancement (I&E)Three (3) lots of product or product family1 week
Chromogenic Assay: RoutineLot size dependent; 1-10 units1 week
Chromogenic Assay: Inhibition and Enhancement (I&E)Three (3) lots of product or product family1 week